Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1984-04-03
1988-08-30
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435806, 436 63, 436906, C12Q 102
Patent
active
047677031
ABSTRACT:
A method for evaluating the fertility of a male mammal. A semen sample is obtained from the individual to be tested. The sperm is separated from the seminal plasma of the semen sample. At least one test portion and a control portion of the sperm are isolated. The sperm portions are incubated, the test portion being exposed to a glycosaminoglycan in a concentration effective to induce an acrosome reaction in sperm. A representative sample of incubated sperm from each portion is then counted by means of observation by light microscopy to measure the increase in acrosome reaction in the test portions as compared to the control portion. A kit is provided for the convenient performance of the method. The kit includes a supply of sterile liquid culture medium, sterile closable culture vessels, and a supply of selected, sterile GAG meterable substantially aseptically in known amounts into each culture vessel used as a test vessel.
REFERENCES:
Schill et al.--Chem. Abst. vol. 86 (1977), p. 184,948v.
Schill et al.--Chem. Abst. vol. 82 (1975), p. 41199h.
Good Pasture--Chem. Abst. vol. 94 (1981), p. 13738n.
J. H. D. Bryan and S. R. Akruk (1977), A Naphthol Yellow S and Erythrosin B Staining Procedure for Use in Studies of the Acrosome Reaction of Rabbit Spermatozoa, Stain Technol., 52, 47-50.
R. C. Jones (1973), Preparation of Spermatozoa for Electron and Light Microscopy, J. Reprod. Fertil., 33, 145-149.
W. Byrd (1981), In Vitro Capacitation and the Chemically Induced Acrosome Reaction in Bovine Spermatozoa, J. Exp. Zool., 215, 35-46.
J. M. Bedford (1970), Sperm Capacitation and Fertilization in Mammals, Biol. Reprod., Suppl. 2, 128-158.
S. Aonuma et al. (1973), Studies on Sperm Capacitation: I. The Relationship Between a Guinea-Pig Sperm-Coating Antigen and a Sperm Capacitation Phenomenom, J. Reprod. Fertil., 35, 425-432.
D. W. Fawcett (1975), The Mammalian Spermatozoon, Dev. Biol., 44, 394-436.
R. Yanagimachi (1978), Sperm-egg Association in Mammals, Curr. Top. Dev. Biol., 12, 83-105.
T. J. Wincek et al. (1979), Fertilization: A Uterine Glycosaminoglycan Stimulates the Conversion of Sperm Proacrosin to Acrosin, Science, 203, 553-554.
R. F. Parrish et al. (1980), Glycosaminoglycan Stimulation of the in vitro Conversion of Boar Proacrosin into Acrosin, J. Androl., 1, 89-95.
R. B. L. Gwatkin and O. F. Andersen (1969), Capacitation of Hamster Spermatozoa by Bovine Follicular Fluid, Nature, (Lond.), 224, 1111-1112.
H. J. Grimek and R. L. Ax (1982), Chromatographic Comparison of Chondroitin-Containing Proteoglycan from Small and Large Bovine Ovarian Follicles, Biochem. Biophys. Res. Comm., 104, 1401-1406.
R. W. Lenz et al. (1982), Proteoglycan from Bovine Follicular Fluid Enhances an Acrosome Reaction in Bovine Spermatozoa, Biochem. Biophys. Res. Comm., 106, 1092-1098.
R. R. Handrow et al. (1982), Structural Comparisons Among Glycosaminoglycans to Promote an Acrosome Reaction in Bovine Spermatozoa, Biochem. Biophys. Res. Comm., 107, 1326-1332.
P. V. Dandekar and M. Gordon (1975), Electron Microscope Evaluation of Rabbit Eggs Exposed to Spermatozoa Treated with Capacitating Agents, J. Reprod. Fertil., 44, 143-146.
K. T. Kirton and H. D. Hafs (1965), Sperm Capacitation by Uterine Fluid or B-Amylase in vitro, Science, 150, 618-619.
A. Rosado et al. (1974), Capacitation in vitro of Rabbit Spermatozoa with Cyclic Adenosine Monophosphate and Human Follicular Fluid, Fertil. Steril., 25, 821-824.
J. M. Bedford (1969), Morphological Aspects of Sperm Capacitation in Mammals, Adv. Biosci., 4, 35-50.
B. G. Brackett and G. Oliphant (1975), Capacitation of Rabbit Spermatozoa in vitro, Biol. Reprod., 12, 260-274.
T. Iwamatsu and M. C. Chang (1969), in vitro Fertilization of Mouse Eggs in the Presence of Bovine Follicular Fluid, Nature, 224, 919-920.
B. D. Bavister and R. Yanagimachi (1977), The Effects of Sperm Extracts and Energy Sources on the Motility and Acrosome Reaction of Hamster Spermatozoa in vitro, Biol. Reprod., 16, 228-237.
W. B. Schill, Fert. Ster. (1974), 25: 703-712.
Goodpasture et al., J. Androl. (1980), 1: 16-27.
Goodpasture et al., J. Reprod. Fert. (1981), 63: 397-405.
Goodpasture et al., J. Androl. (1982), 3: 151-156.
Ax Roy L.
Lenz Richard W.
Houser David J.
Rosen Sam
Wisconsin Alumni Research Foundation
LandOfFree
Method for assessing the fertility of male mammals does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for assessing the fertility of male mammals, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for assessing the fertility of male mammals will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2088021