Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-02-21
2006-02-21
Le, Long (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007100, C435S007210, C435S026000, C435S188000, C435S007720, C435S007900, C436S518000, C436S173000, C514S562000, C530S402000, C530S408000, C530S409000
Reexamination Certificate
active
07001738
ABSTRACT:
Many of the effects of nitric oxide are mediated by the direct modification of cysteine residues resulting in an adduct called a nitrosothiol. A method to detect proteins which contain nitrosothiols involves several steps. Nitrosylated cysteines are converted to tagged cysteines. Tagged proteins can then be detected, for example, by immunoblotting and/or can be purified by affinity chromatography. The method is applicable to the detection of S-nitrosylated proteins in cell lysates following in vitro S-nitrosylation, as well as to the detection of endogenous S-nitrosothiols in selected protein substrates.
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Erdjument-Bromage Hediye
Ferris Christopher D.
Jaffrey Samie R.
Snyder Solomon H.
Tempst Paul
Banner & Witcoff , Ltd.
Davis Deborah A.
Le Long
Memorial Sloan-Kettering Cancer Center
The Johns Hopkins University
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