Method for assaying primary amines, secondary amines and peptide

Details Method for assaying primary amines, secondary amines and peptide Method for assaying primary amines, secondary amines and peptide

1987-03-11

1990-01-02

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436 89, 436 90, 436111, 436172, G01N 2177, G01N 3368

Patent

active

048913235

ABSTRACT:
Primary amines, secondary amines and peptides are fluorometrically assayed using a fluorogenic derivatization reagent of the formula: ##STR1## where Z is an electron withdrawing group or a fused benzene ring, Y and Y', which are identical or different, are nitrogen or carbon and X is a halogen or other leaving group. The above fluorogenic reagent reacts with the primary amine, secondary amine or peptide to form a cyclic fluorescent adduct.

REFERENCES:
patent: 4359323 (1982-11-01), LePage
patent: 4419452 (1983-12-01), Imai et al.
patent: 4670403 (1987-06-01), Ishida et al.
patent: 4758520 (1988-07-01), Matuszewski et al.
Gardner et al, Anal. Biochem, vol. 101, pp. 61-65, 1980.
Larsen et al, J. of Chromatographic Science, vol. 19, pp. 259-265, 1981.
Ishida et al, J. of Chromatography, vol. 204, pp. 143-148, 1981.
Kucera et al, J. of Chromatography, vol. 255, pp. 563-579, 1983.

Affiliated with

Also associated with

Rating

Method for assaying primary amines, secondary amines and peptide does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for assaying primary amines, secondary amines and peptide, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for assaying primary amines, secondary amines and peptide will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1383534