Method for assaying nucleic acids using naphthol derivative phos

Details Method for assaying nucleic acids using naphthol derivative phos Method for assaying nucleic acids using naphthol derivative phos

1991-12-13

1996-01-16

Beisner, William H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 21, 534573, 548113, 558 9, 558193, C12Q 168, C12Q 142

Patent

active

054847001

ABSTRACT:
Methods for detecting nucleic acids in a sample, using naphthol derivative phosphates, are provided. Nucleic acids present in a sample are contacted with phosphatase, producing a modified phosphatase. A naphthol derivative phosphate is contacted with the modified phosphatase to produce a reaction product. Any reaction product formed is detected by irradiating it with an excited light and detecting a fluorescence emitted from the reaction product. Naphthol derivative phosphates useful in these methods are also provided. Methods for production of naphthol derivative phosphates are also provided.

REFERENCES:
patent: 3140283 (1964-07-01), Depoorter
patent: 3180873 (1965-04-01), Schmidt
patent: 4582789 (1986-04-01), Sheldon, III et al.
Vaughan A., Fluorometric Methods for Analysis . . . , Anal Chem 43 (6) May 1971 pp. 721-724.
West S., A Multiple Staining Procedure for the Detection . . . , Anal Chem 190, 1990, pp. 254-258.

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