Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-12-22
1995-01-31
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 21, C12Q 168, C12Q 142, G01N 33533
Patent
active
053858239
ABSTRACT:
Method for assaying nucleic acids and proteins employing no radiosotope labeling, which is suitable for data storage and allows to carry out the detection efficiently and high sensitivity. The sample selectable from the group of nucleic acids, proteins, and other chemical compounds is adhered on a nylon membrane filter to be bound to a phosphatase. Then the phosphatase is reacted with an anthracene derivative phosphate followed by irradiating the reaction product with ultraviolet light to detect a fluorescence emitted therefrom.
REFERENCES:
patent: 4978614 (1990-12-01), Bronstein
Vaughan et al., Analytical Chemistry 43(6), 721-724 (1971).
Schroeder et al., BioEngineering 5(3-4), 24-30 (1989), (Abstract).
Schmeer et al., J. Clin. Microbiol. 26(12), 2520-2525 (1988).
Coutlee et al., J. Clin. Microbiol. 27(5), 1002-1007 (1989).
"A Comparison of Chemiluminescent and Colorimetric Substrates in a Hepatitis B Virus DNA Hybridization Assay", by Irena Bronstein et al., Annalytical Biochemistry, vol. 180:95-98 (1989).
"A Fluorescent Detection Method for DNA Hybridization Using 2-Hydroxy-3-Naphthoic Acid-2'-Phenylanilide Phosphate as a Substrate For Alkaline Phosphatase", by Naoto Kagiyama et al., Acta Histochem. Cytochem., vol. 25, No. 4, pp. 467-471, (1992).
"Non-radioactive Labeling and Detection of Nucleic Acids", by Christopher Kessler et al., Bio. Chem. Hoppe Seyler, vol. 371, pp. 917-927, (Oct. 1990).
Fujita Satoshi
Hori Hiroshi
Kagiyama Naoto
Kondo Yasumitsu
Momiyama Masayoshi
Aisin Seiki Kabushiki Kaisha
Horlick Kenneth R.
Parr Margaret
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