Method for assaying nucleic acids

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435772, 435 21, 548113, C12Q 168, C12Q 142

Patent

active

055321259

ABSTRACT:
A method for assaying nucleic acids or similar compounds comprises binding a sample such as a nucleic acid to phosphatase; reacting the phosphatase with a 3-hydroxy-2-naphthoic acid-2'-phenyl anilide phosphate; irradiating the reaction product with an excited light; and detecting fluorescence emitted therefrom.

REFERENCES:
patent: 3140283 (1964-07-01), Depoorter et al.
patent: 3180873 (1965-04-01), Schmidt et al.
patent: 4582789 (1986-04-01), Sheldon, III et al.
patent: 4617261 (1986-10-01), Sheldon, III et al.
patent: 4705886 (1987-11-01), Levenson et al.
patent: 4751313 (1988-06-01), Levenson et al.
patent: 4754065 (1988-06-01), Levenson et al.
patent: 4803297 (1989-02-01), Levenson et al.
patent: 4889798 (1989-12-01), Rabbani
Burstone, M. S., Enzyme Histochemistry and Its Application in the Study of Neoplasms, pp. 64-67, 82-83 (Academic Press, 1962).
Chemical Abstracts, 55, abstract No. 10550 (g) (1961).
Chemical Abstracts, 115(25): abstract No. 275243v (1991).
Mullink, H., et al., "Combined Immuno-and Non-radioactive Hybridocyto-chemistry on Cells and Tissue Sections: Influence of Fixation, Enzyme Pre-treatment, and Choice of Chromogen on Detection of Antigen and DNA Sequences," The Journal of Histochemistry and Cytochemistry, 37(5): 603-609 (1989); Chemical Abstracts, 110: 228120q (1989).
Rompp Chemie Lexikon, pp. 3375-3376 (9th ed.) Date Not Avail.
Vaughan, A., et al., "Fluorometric Methods for Analysis of Acids and Alkaline Phosphatase," Analytical Chemistry, 43(6): 721-724 (1971).
West, S., et al., "A Multiple-Staining Procedure for the Detection of Different DNA Fragments on a Single Blot," Analytical Biochemistry, 190: 254-258 (1990).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for assaying nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for assaying nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for assaying nucleic acids will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1505689

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.