Method for assaying glycated protein

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S024000, C435S975000

Reexamination Certificate

active

07070948

ABSTRACT:
Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an α-glycated peptide, particularly preferably an α-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.

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Nobuyuld Yoshida, et al., “Primary structures of funga fructosyl amino acid oxidases and their application to the measurement of glycated proteins”, European Journal of Biochemistry, Berlin, DE, vol. 242, No. 3, Dec. 15, 1996, pp. 499-505.
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