Method for assaying genetic attributes in cotton fiber cells

Details Method for assaying genetic attributes in cotton fiber cells Method for assaying genetic attributes in cotton fiber cells

1998-06-30

2000-12-26

Fox, David T.

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

800294, 800278, 800287, 435427, 435469, 536 236, 536 241, C12N 1582, C12N 1584, C12N 504, C12N 1529, A01H 400

Patent

active

061663018

ABSTRACT:
The present invention provides methods for testing gene expression in a cotton fiber cells. The methods comprise contacting the cell with Agrobacterium sp., comprising a recombinant T-DNA vector, which includes a plant promoter operably linked to a gene of interest; and detecting the product of the polynucleotide of interest, thereby testing for expression of the polynucleotide of interest.

REFERENCES:
patent: 5004863 (1991-04-01), Umbeck
patent: 5159135 (1992-10-01), Umbeck
patent: 5602321 (1997-02-01), Maliyakal
Shen, Wen-Hui, et al.: "T-DNA transfer to maize cells: Histochemical investigation of .beta.-glucuronidase activity in maize tissues", Proc. Natl. Acad. Sci USA, 90:1488-1492 (1993).
John, Maliyakal E. "Cotton Crop Improvement Through Genetic Engineering", Critical Reviews in Biotechnology, 17(3):185-208 (1997).
Hansen, Genevieve, et al.: "Constitute expression of the virulence genes improves the efficiency of plant transformation by Agrobacterium", Proc. Natl. Acad. Sci USA, 91:7603-7607 (1994).
Umbeck et al. Biotechnology. 1987. vol. 5: 263-266.

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