Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Glycoproteins
Patent
1990-11-30
1991-12-31
Hill, Jr., Robert J.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
Glycoproteins
422 56, 436 86, 436163, 436169, G01N 2178, G01N 3368
Patent
active
050772228
ABSTRACT:
A test device and method of determining the presence and concentration of proteins, such as albumin or Bence Jones proteins, in a test sample. The test device includes a carrier matrix incorporating a reactant system capable of interacting with proteins to produce a visually or instrumentally detectable and/or measurable response. The carrier matrix of the device can include commonly used bibulous matrices, such as filter paper, or a nonbibulous protein-permeable strip, membrane or layer of a polymerized urethane-containing composition. In addition, a reactant system, including a dual indicator reagent system, such as bromophenol blue, methyl orange and, if necessary, a suitable buffer, is incorporated into the carrier matrix to provide improved color resolution and increased sensitivity to proteins, thereby affording a more accurate and trustworthy protein assay of test samples, such as urine. Furthermore, by incorporating the dual indicator reagent system into a carrier matrix including a polymerized urethane-containing composition, a dry phase assay for low molecular weight proteins, such as Bence Jones proteins, can be performed on a liquid test sample.
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Coe Roger N.
Hill Jr. Robert J.
Miles Inc.
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