Method for assaying ERK2 map kinase

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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4241391, 4241381, 435 723, 5303879, 5303877, G01N 3353, G01N 33574, A61K 39395, C07K 1600

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active

060015802

ABSTRACT:
The monoclonal antibody generated by immunizing a mammal with human MAP kinase ERK1 and the polyclonal antibody generated by immunizing an animal with the phosphorylation region of the sequence of MAP kinase or a fragment of human MAP kinase are used either alone or in combination to detect and assay various MAP kinase species including ERK2.

REFERENCES:
F. A. Gonzalez et al., Heterogeneous Expression of Four MAP Kinase Isoforms in Human Tissues, FEBS 304(2, 3):170-178 (1992).
J. Gomez-Cambronero et al., Granulocyte-Macrophage Colony-Stimulating Factor-Induced Protein Tyrosine Phosphorylation of Microtuble-Associated Protein Kinase in Human Neutrophils, Proc. Natl. Acad. Sci. 89:7551-7555 (1992).
S. J. Cook et al., RapV12 Antagonizes Ras-Dependent Activation of ERK1 and ERK2 by LPA and EGF in Rat-1 Fibroblasts, EMBO 12(9):3475-3485 (1993).
D. L. Charest et al., Molecular Cloning, Expression, and Characterization of the Human Mitogen-Activated Protein Kinase p44ERK1, Mol. Cell. Biol. 13(8):4679-4690 (1993).
M. G. Anderson et al., Requirement for Integration of Signals from two Distinct Phosphorylation Pathways for Activation of MAP Kinase, Nature 343:651-653 (1990).
F. Ito et al. Production of Various Anti MAP Kinase Antibodies, The Japanese Biochemical Society, 66(7):1068, Abstract 4887 (1994) (English translation attached).
Anti Mouse MAP Kinase (erk2), Monoclonal Antibody, Life Science Reagents 3rd ed., p. 737 (1995/1996) (English translation attached).
Campos-Gonzales et al, "Temperature-dependent tyrosine phoshorylation of microtubule-associated protein kinase in epidermal growth factor-stimulated human fibroblasts"Cell Regul. vol. 2, No. 8, pp. 663-674, Aug. 1991.
Affiniti Research Products Limited, "Data Sheet", 1994.
English Abstract of Japanese Publication No. 8-5632, 1996.

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