Method for assaying components in an enzyme-protein substrate sy

Details Method for assaying components in an enzyme-protein substrate sy Method for assaying components in an enzyme-protein substrate sy

1996-08-21

1998-07-28

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 13, 435 23, 435 24, 435 18, 435968, 436536, 436546, 436 69, 436 63, 436800, C12Q 100, C12Q 156

Patent

active

057861374

ABSTRACT:
Assays and reagents are provided for the measurement of components that are involved in either an enzyme-catalyzed degradation of a protein substrate, or an enzyme-catalyzed polymerization of a substrate. The method involves using a fluorescent-labeled protein substrate in an enzyme-catalyzed reaction, and measurement of the component's effects on the fluorescence emission of the enzyme-catalyzed reaction such as quenching or dequenching, as a measure of the component's activity.

REFERENCES:
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patent: 5071745 (1991-12-01), Triscott et al.
patent: 5567596 (1996-10-01), Diamond et al.
Gallimore, et al., "Chromogenic Peptide Substrate Assays and Their Clinical Applications", Blood Reviews, vol. 5, pp. 117-127, 1991.

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