Method for assaying bilirubin .delta. fractions

Details Method for assaying bilirubin .delta. fractions Method for assaying bilirubin .delta. fractions

1998-02-26

1999-08-31

Weber, Jon P.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

C12Q 126

Patent

active

059452986

ABSTRACT:
By making a bilirubin oxidase derived from the genus Pleurotus to act on a living body fluid sample in the presence of at least one of a cationic surfactant such as alkyltrimethylammonium salt, etc. and a nonionic surfactant such as polyoxyethylenealkyl ether, etc., only bilirubin non-.delta. fractions of the living body fluid sample can be oxidized, while keeping a bilirubin .delta. fraction remaining as unreacted. Thus, the bilirubin non-.delta. fractions and .delta. fraction of the living body fluid sample can be exactly quantitatively determined by action of the bilirubin oxidase on the sample.

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