Method for assaying a biocomponent using 4-methoxy-1-naphthol an

Details Method for assaying a biocomponent using 4-methoxy-1-naphthol an Method for assaying a biocomponent using 4-methoxy-1-naphthol an

1985-06-24

1988-04-19

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435 28, 435805, 436516, 436904, G01N 33561, G01N 33535

Patent

active

047389206

ABSTRACT:
The present invention provides a method for assaying a biocomponent on the basis of an amount of bound peroxidase by the steps of supporting a biocomponent or a target substance containing a biocomponent on a gel-like carrier, developing the biocomponent or the target substance by electrophoresis, binding a receptor to the biocomponent or the target substance, binding peroxidase to the receptor, and determining the amount of the bound peroxidase, wherein hydrogen peroxide and 4-methoxy-1-naphthol are used as substrates.

REFERENCES:
patent: 3983005 (1976-09-01), Goodhue
patent: 4200508 (1980-04-01), Hirai
patent: 4235960 (1980-11-01), Sasse
patent: 4272504 (1981-06-01), Kim
patent: 4446240 (1984-05-01), Nerenberg
Taketa Electrophoresis 1983, 4, pp. 371-373.

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