Method for assay of prostaglandins

Details Method for assay of prostaglandins Method for assay of prostaglandins

1974-10-07

1976-01-27

Reese, Robert M.

Chemistry: physical processes

Physical processes

Crystallization

424 7, G01n 2100, G01n 3108, G01n 3316

Patent

active

039349780

ABSTRACT:
A method for assaying prostaglandins of the E and F series comprising the sequential steps of reducing the keto groups in a sample containing the prostaglandins to hydroxy groups, selectively oxidizing the prostaglandin 15-OH groups to keto groups, reducing the keto groups to hydroxy groups by means of a radioactive labeled chemical reducing agent, isolating radioactive labeled prostaglandins and measuring the amount of radioactivity of the isolated radioactive labeled prostaglandins.

REFERENCES:
N. Ambache et al., Brit. J. Pharmacol. 33, 162, (1968).
E. W. Horton, Physiological Review 49, 122, (1969).
N. H. Andersen, Chem. Abstr. 71, 27642p (1969).
E. Anggard et al., J. Chromatogr. 48, 542, (1970).

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