Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1985-06-14
1988-10-25
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435803, 435810, C12Q 128
Patent
active
047802812
ABSTRACT:
A preferred method wherein 5-phenyl-4-pentenyl-hydroperoxide (PPHP) is reduced to 5-phenyl-4-pentenyl-alcohol (PPA) by plant and animal peroxidases in the presence of reducing substrates is described. The method also uses related homologs containing 3 to 8 carbon atoms. PHP and PPA are rapidly isolated with solid phase extraction, separated by isocrated reverse phase high performance liquid chromatography, and quantitated with a fixed-wavelength ultraviolet detector. The procedure described in suitable for detecting peroxide reducing enzymes, determining the kinetic properties of heme- and non-heme-containing peroxidases, and evaluating oxidizable compounds as reducing substrates for peroxidases. The method identifies compounds which are reducing substrates and also ranks them for relative activity. The method can be used to identify active antithrombotic, antimetastatic, or anti-inflammatory drugs as substrates as well as detect and characterize mammalian peroxidases.
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Marnett Lawrence J.
Weller Paul E.
Herald William J.
McLeod Ian C.
Warden Robert J.
Wayne State University
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