Combinatorial chemistry technology: method – library – apparatus – Method of screening a library – By measuring the ability to specifically bind a target...
Reexamination Certificate
2011-08-09
2011-08-09
Wessendorf, T. D. (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Method of screening a library
By measuring the ability to specifically bind a target...
C435S007210, C435S007200, C435S007100, C435S004000, C506S007000
Reexamination Certificate
active
07994096
ABSTRACT:
The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.
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Faulhaber Nicole
Kern Florian
Khatamzas Elham
Reinke Petra
Surel Ingolf-Pascal
Norris McLaughlin & Marcus P.A.
Wessendorf T. D.
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