Method for analyzing the glycation of hemoglobin

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

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435 4, 435 14, 436 67, A01N 102, C12Q 100, C12Q 154, G01N 3372

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053842391

ABSTRACT:
As red blood cells age, their hemoglobin is progressively glycosylated. This invention relates to analyzing the history of blood sugar metabolism over a period of several months by taking a blood sample, separating the cells into age-ordered cohorts, determining glycohemoglobin levels in the cells of each cohort, and correlating glycohemoglobin levels with red blood cell age, while correcting for the reversibility of the Amadori rearrangement.

REFERENCES:
patent: 4835097 (1989-05-01), Saunders
Proceedings, (Published by ISOLAB, INC.) The Role of Glycated Hemoglobin in the Management of Diabetes, Symposium, Sep. 16, 1988, pp. 9-19 and 51-72.
Mortensen, Glycated Hemoglobin, Reaction and Biokinetic Studies Clinical Application of Hemoglobin A.sub.1c in the Assessment of Metabolic Control in Children with Diabetes Mellitus, Danish Medical Bulletin 32(6):309-328 (Dec. 1985).
Beach, Kirk W., "A Theoretical Model to Predict the Behavior of Glycosylated Hemoglobin Levels", The Journal of Theoretical Biology, 81:3:547-561, (1979).
Elseweidy, et al., "Changes in glycosylated hemoglobine with red cell aging in normal and diabetic subjects and in newborn infants of normal and diabetic mothers", The Journal of Laboratory and Clinical Medicine, 102:1:628-636, (Jul. 1983).
Bunn, H. F. et al. "The Biosynthesis of Human Hemoglobin A.sub.1c Slow Glycosylation of Hemoglobin In Vivo", The Journal of Clinical Investigation, 57:1652-1659 (1976).
Higgins, P. J. et al. "Kinetic Analysis of the Nonenzymatic Glycosylation of Hemoglobin", The Journal of Biological Chemistry, 256(10):5204-5208 (May 25, 1981).
Nakashima, K. et al., Clin. Chem. 35:958-962 (1989).

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