Method for analyzing protein

Details Method for analyzing protein Method for analyzing protein

2006-10-18

2011-11-22

Noguerola, Alex (Department: 1759)

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

C204S450000

Reexamination Certificate

active

08062492

ABSTRACT:
A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I:wherein R1is an aryl group or a heteroaryl group that may be substituted, R2is a heterocyclic group that may be substituted, and n is an integer between 1 and 5,is dissolved, and then detecting a complex of the compound of formula I and the protein via spectrophotometrical measurement.

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Suzuki et al., Design and Synthesis of Intramolecular Charge Transfer-Based Fluorescent Reagents for the Highly-Sensitive Detection of Proteins, JACS, 2005,127, 17799-17802.
Suzuki, Yoshio et al., “Design and Synthesis of Intramolecular Charge Transfer-Based Fluorescent Reagents for the Highly-Sensitive Detection of Proteins”, Journal of the American Chemical Society, vol. 127, No. 50, pp. 17799-17802, (2005).

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