Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1995-01-27
1995-10-17
Niebling, John
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
2041828, 2041801, G01N 2726, G01N 27447
Patent
active
054587611
ABSTRACT:
Analysis of a nucleic acid samples having thousands of bases is conducted by capillary electrophoresis. The electrophoretic section is provided with a first capillary filled with an agarose gel and a second capillary filled with a polyacrylamide gel. An on-column detector is incorporated with the second capillary for optical detection. To fill a capillary with a gel, a solution is fed under high pressure from a first flow channel through a switching valve into a second flow channel connected to the capillary. To inject a sample in the capillary, a sample injector is connected to a switching valve passage, and a buffer solution is connected to the capillary through a flow channel and the switching valve. After switching the valve, the first passage is incorporated into the flow channel between the buffer solution and the capillary being filled. Then, the sample is electro-kinetically injected into the capillary. When conducting genetic polymorphism by electrophoresis, temperature control elements are provided to maintain the capillary at a predetermined temperature and a DNA sample device is provided to heat the sample to a temperature higher than a disassociation temperature thereof for directly injecting the heated sample into the capillary.
REFERENCES:
patent: 3847785 (1974-11-01), Allington
patent: 4810456 (1989-03-01), Bente, III et al.
patent: 4859301 (1989-08-01), Brenner et al.
patent: 4898658 (1990-02-01), Karger et al.
patent: 5085756 (1992-02-01), Swedberg
patent: 5085757 (1992-02-01), Karger et al.
patent: 5110424 (1992-05-01), Chin
patent: 5240576 (1993-08-01), Lauer et al.
Ewald J. B. M. Mensink et al "Detection of point mutations in DNA using capillary electrophoresis in a polymer network" Journal of Chromatography, vol. 621, No. 2 (24 Nov. 1993) 149-156.
Takashi Satow et al "Simultaneous determination of the migration coefficient of each base in heterogeneous oligo-DNA by gel filled capillary electrophoresis" Journal of Chromatography A, vol. 652, No. 1 (15 Oct. 1993) 23-30.
Science, vol. 222, Oct. 1983, pp. 266-272. James W. Jorgenson et al "Capillary Zone Electrophoresis".
Analytical Chemistry, May 1990, 62, pp. 900-903, Lloyd M. Smith et al "High Speed Separations of DNA Sequencing Reactions by Capillary Electrophoresis".
Journal of Chromotography, 516, 1990, 33-48, Barry L. Karger et al, "Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electrophoresis".
Proceedings of National Academy of Science of USA, 86, 1989, pp. 2766-2770, Takao Sekiya et al, "Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms".
Analytical Chemistry, 64, Jun. 1992, pp. 1221-1225, Yoshinobu Baba et al, "Preparation of Polyacrylamide Gel Filled Capillaries for Ultrahigh Resolution of Polynucleotides by Capillary Electrophoresis".
Journal of Chromotography, 559, 1991, pp. 267-283. Herbert E. Schwartz et al. "Analysis of DNA restriction fragments and polymerase chain reaction products towards detection of the AIDS (HIV.1) virus in blood".
Journal of Chromotography, 559, 1991, pp. 295-305, Barry L. Karger et al "Identification of DNA molecules by pre-column hybridization using capillary electrophoresis".
Gobinda Sarkar et al, "Screening for Mutations by RNA Single-Strand Conformation Polymorphism (RSSCP): Comparison with DNA-SSCP,", Nucleatic Acids Research, 1992, 20(4), pp. 871-878.
"Isolation of High-Molecular-Weight, Eukaryotic DNA From Cells Grown in Tissue Culture," Construction of Genomic Libraries, 1982, pp. 280-281.
Fujita Takeshi
Kamahori Masao
Umemura Shin'ichiro
Yamada Takashi
Hitachi , Ltd.
Niebling John
Starsiak Jr. John S.
LandOfFree
Method for analyzing nucleic acid or protein and apparatus there does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for analyzing nucleic acid or protein and apparatus there, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for analyzing nucleic acid or protein and apparatus there will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-594136