Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2003-07-24
2009-12-22
Schultz, JD (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100, C536S024300, C536S025300, C204S451000
Reexamination Certificate
active
07635558
ABSTRACT:
The invention concerns a method for analyzing nucleic acids using a small-size probe array comprising deoxyinostines (dI) instead of deoxyguanogines (dG). The invention also concerns such probe arrays and their use in methods for detecting and/or quantifying target oilgonucleotides present in DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) molecules in a sample, in particular mRNA editing rate of the serotonin 5-HT2Creceptor (5-HT2C-R). The invention further concerns a biochip or a reactor in liquid medium comprising such probe arrays as well as their uses, in particular for detecting and/or identifying genetic polymorphisms or for determining an mRNA editing rate, whether it is that of a 5-HT2C-R mRNA or any other RNA capable of being edited. The invention also concerns a method based on the isolation of a single strand conformation polymorphism (SSCP) enabling under specific analysis conditions the editing profile and/or rate of an mRNA capable of being edited to be obtained, as well as a method for diagnosing diseases or susceptibility to diseases associated with the degree of edition of an mRNA. Finally, the invention concerns a method for selecting compounds capable of modulating mRNA editing rate, in particular that of 5-HT2C-R, as well as the use of such compounds for preparing a pharmaceutical composition for treating organic fluid.
REFERENCES:
patent: 5487972 (1996-01-01), Gelfand et al.
patent: 5578467 (1996-11-01), Schuster et al.
patent: 6156501 (2000-12-01), McGall et al.
patent: 2001/0034023 (2001-10-01), Stanton et al.
patent: 0 552 931 (1993-07-01), None
patent: WO 02 38809 (2002-05-01), None
Larsen et al, High throughput single strand conformation polymorphism analysis, 1999, Huamn mutation, 13, 318-327.
International Search Report in Corresponding PCT application No. PCT/FR03/02339.
Niswender et al., Identification and characterization of RNA editing events within the 5-HT2Creceptor,Annals of the New York Academy of Sciences, vol. 861, pp. 38-48 (1998).
Nguyen et al., Studies towards the design of a modified GC base pair with stability similar to that of the AT base pair,Tetrahedron Letters, vol. 38, No. 23, pp. 4083-4087 (1997).
Niswender, Coleen, Strategies and requirements for the detection of RNA editing in G protein coupled-receptor RNA,Methods in Enzymology, vol. 343, pp. 476-492 (2002).
Zhong et al., Detection of apolipoprotein B mRNA editing by peptide nucleic acid mediated PCR clamping,Biochemical and Biophysical Research Communications, vol. 259, pp. 311-313 (1999).
Fuchs et al., RNA editing in higher plant plastids: oligoribonucleotide SSCP analysis allows the proof of base conversion directly at the RNA level,Current Genetics, vol. 39, No. 5-6, pp. 384-387 (Jul. 2001).
Ibrahim, et al., Differential expression of potato U1A spliceosomal protein genes: a rapid method for expression profiling of multigene families,Plant Molecular Biology, vol. 45, No. 4, pp. 449-460 (Mar. 2001).
Maekawa, et al., Relative ratios of mRNA molecules encoded by genes with homologous sequences using fluorescence-based single-strand conformation polymorphism analysis;Biochemical and Biophysical Research Communications, vol. 223, No. 1, pp. 520-525 (1996).
Ellison, Jane, Fluorescence-based mutation detection,Molecular Biotechnology, vol. 5, No. 1, pp. 17-31 (1996).
Berthomme Hervé
Madjar Jean-Jacques
Bhat Narayan K
Biocortech
Finnegan Henderson Farabow Garrett & Dunner L.L.P.
Schultz JD
LandOfFree
Method for analyzing nucleic acid and use thereof for... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for analyzing nucleic acid and use thereof for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for analyzing nucleic acid and use thereof for... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4131731