Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-10-23
2000-07-11
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, C12P 1934, C12Q 168
Patent
active
060871342
ABSTRACT:
Methods are provided for analyzing DNA of a rare cell in a cell population. In one embodiment, the method involves covering a cell monolayer with a photosensitive material. By illuminating the area over a cell of interest, the material is solidified, permitting manipulation of the underlying cell and/or protection of the cell from DNA-inactivating agents that destroy DNA in other cells in the monolayer. In another embodiment, the monolayer is overlaid with a solid material that becomes soluble when illuminated. By illuminating the area over a cell of interest, that cell can be specifically exposed and DNA from the cell amplified. The methods are particularly useful for analyzing fetal cells found in maternal blood.
REFERENCES:
patent: 3715293 (1973-02-01), Sander et al.
patent: 3801329 (1974-04-01), Sander et al.
patent: 4545831 (1985-10-01), Ornstein
patent: 4624915 (1986-11-01), Schindler et al.
patent: 4629687 (1986-12-01), Schindler et al.
patent: 5432054 (1995-07-01), Saunders et al.
Marilyn J. Stapleton (1996) Genetic Engineering News p. 14 Sep. 1, 1996 Gene Amplification for Human Diagnostics.
Michael R. Emmert-Buck et al. (1996) Science 274:998 Laser Capture Microdissection.
C. G. Roffey (1982) Photopolymerization of Surface Coatings Chapter 4, John Wiley & Sons, New York Photopolymerizable Film-Forming Materials.
C. G. Roffey (1982) Photopolymerization of Surface Coatings Chapter 6, John Wiley & Sons, New York Photopolymerizable Film-Forming Materials.
Pierce Catalog and Handbook (1994-1995) pp. 0-92, T-155, T-156, T-157, T-163 T-164, T-165, T-169, T-170, T-174 and T-176.
Emmer-Buck et al. Laser capture microdissection. Science, vol. 274 (8), p. 998-1001, 1996.
Zekizawa et al. Prenatal diagnosis of Duchenne muscular dystrophy using a single fetal nucleated erythrocyte in maternal blood. Neurology vol. 46, pp. 1350-1353, 1996.
Vaigot et al, Cytometry 6(5): 422-7, 1985.
Heiden et al Virchos Arch B Cell Path 61(1): 29-38, 1991.
Applied Imaging Corporation
Campbell Eggerton A.
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