Method for analyzing biologically active substances

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 71, 435 4, 536 243, 935 77, 935 78, C12Q 168, C12Q 100, C07H 2104

Patent

active

059087463

ABSTRACT:
A method is provided, comprising the steps of reacting a biologically active first substance immobilized on a carrier with a second substance capable of specifically binding the first substance, and detecting a non-bound part of the second substance or a bound part of the second substance indirectly bound to the carrier through binding between the first and second substances so that the first substance or the second substance in a sample is analyzed, wherein the carrier carries a compound having 2 to 100 carbodiimide groups, and the first substance is immobilized on the carrier through the carbodiimide groups so that the active substance such as protein and nucleic acid is bound to the carrier conveniently, efficiently, and tightly.

REFERENCES:
patent: 3929733 (1975-12-01), Alberino et al.
patent: 4280992 (1981-07-01), Sugiura et al.
patent: 5104791 (1992-04-01), Abbott et al.
patent: 5122600 (1992-06-01), Kawaguchi et al.
patent: 5514785 (1996-05-01), Van Ness et al.
patent: 5582970 (1996-12-01), Wallace
patent: 5622826 (1997-04-01), Varma
Zhang et al. Nucleic Acids Research 19(14): 3929-3933 (Jul. 25, 1991).
Arrand et al. in Nucleic Acid Hybridization, Hames et al editors. IRL Press, Washington DC (1985 / pp. 17-45).
Patent Abstracts of Japan, JP 04 021637 (Soken Kagaku KK) Jan. 24, 1992). Abstract only.
Desai et al., "Polymer Bound EDC (P-EDC); A Convenient Reagent for Formation of an Amide Bond", Tetrah. Ltrs., vol. 34, No. 48, pp. 7685-7688, 1993.
Rasmussen, et al., "Covalent Immobilization of DNA onto Polystyrene Microwells: The Molecules are Only Bound at the 5' End", Analy. Biochem., vol. 198, pp. 138-142 (1991).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for analyzing biologically active substances does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for analyzing biologically active substances, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for analyzing biologically active substances will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-954442

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.