Radiant energy – Ionic separation or analysis – With sample supply means
Patent
1991-01-31
1993-05-11
Anderson, Bruce C.
Radiant energy
Ionic separation or analysis
With sample supply means
250287, 250282, B01D 5944, H01J 4940
Patent
active
052104125
ABSTRACT:
Described is a method and apparatus for analyzing an organic sample. In the preferred embodiment, this method and apparatus allows the determination of the base sequence of a nucleic acid by determining the molecular weights of the components of a biological sample. The method uses either a pre-existing chromophore or the covalent attachment of an ionizable chromophore to a biological sample followed by the vaporization of these molecules by exposure to an intense pulse of electromagnetic radiation in the presence of a matrix which strongly absorbs the radiation. The gaseous molecules are subsequently extracted into an evacuated ionization chamber and then exposed to electromagnetic radiation at a wavelength which specifically excites the chromophore covalently attached to the biological sample. The molecular weights of these ionized species are then determined by mass spectroscopic analysis. This method of molecular weight determination allows for a DNA sequencing method. Four samples of DNA molecules are prepared such that each is covalently linked to an ionizable chromophore and each is fragmented by a means which results in fragments within each of the four samples that terminate at a different one of the nucleotides A, C, G or T. Each of these four fragmented samples is subsequently introduced into the said apparatus in order to determine their molecular weights hence providing the strand length of the fragments. The data generated may be analyzed by high-speed computer, the four data sets correlated, and the sequence deduced.
REFERENCES:
patent: 4220414 (1980-09-01), Barringer
patent: 4598577 (1986-07-01), Jowitt et al.
patent: 4678326 (1987-07-01), Harjunmaa
patent: 4680275 (1987-07-01), Wagner et al.
patent: 4681859 (1987-07-01), Kramer
patent: 4731532 (1988-03-01), Frey et al.
patent: 4751190 (1988-06-01), Chipetta et al.
patent: 4757141 (1988-07-01), Fung et al.
patent: 4760258 (1988-07-01), Gast et al.
patent: 4772563 (1988-09-01), Evangelista et al.
patent: 4777128 (1988-10-01), Lippa
patent: 4811218 (1989-03-01), Hunkapiller et al.
patent: 4832815 (1989-05-01), Cambara et al.
patent: 4848904 (1989-07-01), Sapp et al.
patent: 4852017 (1989-07-01), Hunkapiller
patent: 4855225 (1989-08-01), Fung et al.
patent: 4920264 (1990-04-01), Becker
patent: 4927265 (1990-05-01), Brownlee
patent: 4988879 (1991-01-01), Zarc et al.
patent: 5002868 (1991-03-01), Jacobson et al.
patent: 5003059 (1991-03-01), Brennan
patent: 5045694 (1991-09-01), Beavis et al.
"Electrospray Ionization--Principles and Practice", Fenn, et al, Mass Spectrometry Reviews, 1990, 9, 37-70.
"Pulsed High-Pressure Liquid Injection of Biological Molecules, etc.", Pang, et al, Applied Spectroscopy, vol. 42, No. 7, 1988, pp. 1200-1206.
"Volatilization of High Molecular Weight DNA by Pulsed Laser Ablation of Frozen Aqueous Solutions", Nelson, et al, Science, vol. 246, pp. 1585-1587, Dec. 22, 1989.
"Time-of-flight Mass Spectrometry: An Increasing Role in the Life Sciences", Robert J. Cotter, Biomedical & Environmental Mass Spectrometry, vol. 18, pp. 513-532 (1989).
Science/Technology, Human Genome Project Challenges Analytical Community Ward Worthy, C & EN Chicago, Sep. 1990.
Photon Resonance Mass Spectrometry John Wronka Spectroscopy, vol. 5, #3, 1987.
Biomolecules in the Gas Phase: Multiphoton Ionization Mass Spectrometry Jurgen Grotemeyer and Edward W. Schlag Acc. Chem. Res. vol. 22 Nov. 1989.
Secondary Ion Mass Spectrometry with Cesium Ion Primary Beam and Liquid Target Matrix for Analysis of Bioorganic Compounds, Aberth et al., Anal. Chem. vol. 54, Oct. 1982.
Nucleic Acid Related Compounds. 39. Efficient Conversions of 5-Iodo to 5-Alkynyl and Derived 5-Substituted Uracil Bases and Nucleosides Morris Robins Philip J. Barr, J. Org. Chem., vol. 48, 1983.
Forks and Combs and DNA: the Synthesis of Branched Oligodeoxyribonucleotides Thomas Horn and Mickey S. Urdea Nucleic Acid Research vol. 17, 1989.
Ultraviolet-Laser Desorption/Ionization Mass Spectrometry of Femtomolar Amounts of Large Proteins M. Karas, A. Ingendoh, U. Bahr and F. Hillenkamp Biomedical & Environmental Mass Spectr. vol. 18, 1989.
Lasers and Mass Spectrometry edited by David M. Lubman Oxford Univ. Press, 1990, pp. 353-382.
A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides by James M. Prober et al., Science, vol. 238, Oct. 1987.
Mass Spectrometry by: A. L. Burlingame, D. S. Millington, D. L. Norwood and D. A. Russell Anal. Chem. 1990.
Cf-Plasma desorption mass spectrometry by: Bo Sundqvist and Ronald D. Macfarlane, Mass Spectr. Reviews 1985.
Mass Spectrometry and Its Use in Tandem with Laser Spectroscopy by: E. R. Grant and R. G. Cooks, Science, vol. 250, Oct.1990.
Enzymatic Synthesis of biotin-labeled polynucleotides: Novel Nucleic Acid Affinity Probes by: Pennina Langer, Alex Waldrop and David Ward Proc. Natl. Acad. Sci. USA, vol. 78, Nov. 1981.
Resonance Enhanced Multiphoton Ionization of Nucleosides by Using Pulsed-Laser Desorption in Supersonic Beam Mass Spectrometry by Liang Li and David M. Lubman; International J. Mass Spectr. & Ion Process 1989.
Biomolecules in the Gas Phase. III. Multiphoton Ionization Mass Spectra of Phenylthiohydantion Amino Acids and Free Amino Acids by Jurgen Grotemeyer, Klaus Walter, Ulrich Boesl and Edward W. Schlag, Int. Journ. of Mass Spectr. Ion Process, 1987.
"Molecular Weight Determination of Underivatized Oligodeoxyribonucleotides, etc.", Rapid Communications in Mass Spectrometry, vol. 4, No. 4, 1990.
"Electronic Spectroscopy of Small Tryptophan Peptides in Supersonic Molecular Beams", Cable, et al, Journal of the American Chemical Society, 1988, 110, pp. 7349-7355.
Levis Robert J.
Romano Louis J.
Anderson Bruce C.
Wayne State University
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