Method for analyzing amino acids and apparatus therefor

Details Method for analyzing amino acids and apparatus therefor Method for analyzing amino acids and apparatus therefor

1991-11-26

1993-08-17

Housel, James C.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 86, 436 90, 436161, 436178, 210656, 210635, 2101982, 422 70, B01D 1508, G01N 3032

Patent

active

052368475

ABSTRACT:
A method for analyzing amino acids in biological liquids wherein a buffer liquid including unknown components to be analyzed is introduced to a separation column for separating amino acids. After reaction of the buffer liquid in a reactor, the amino acids are detected by a photometer. The flow rate of the buffer liquid is maintained at a predetermined value until the asparagine, glutamine acid and glutamine are separated from the separation column and the flow rate of the buffer liquid is varied stepwise or in linear gradient after separation of the above three components from the separation column.

REFERENCES:
patent: 4133753 (1979-01-01), Takeuchi
Willard, Hobart. "Liquid Column Chromotography." Instrumental Methods of Analysis. 6th ed. New York: D. Van Nostrand Co., 1981.

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