Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1998-03-17
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435911, 435912, 536243, 536 2431, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104
Patent
active
057285268
ABSTRACT:
A method for analyzing a target nucleotide sequence which exists in a first state or a different second state which makes the method particularly useful for determining point mutations. The method uses a first polynucleotide which is immobilized on a solid support and which is at least partially complementary to a first segment of the target nucleotide sequence. By means of a series of steps, a product of the first polynucleotide and a further polynucleotide that contains a detectable label can be obtained. When the state to be analyzed occurs in a rare population, amplification can be conducted so that substantially only amplification of the target nucleotide sequence in one of the states is attained. The method can be used to analyze multiple target sequences simultaneously. A kit which can be used in the method is also set forth.
REFERENCES:
patent: 4656127 (1987-04-01), Mundy
patent: 4683202 (1987-07-01), Mullis
patent: 4988617 (1991-01-01), Landegren et al.
patent: 5427930 (1995-06-01), Birkenmeyer et al.
Debuire et al, "Fast, Manual, Nonradioactive method for DNA sequencing", Clin. Chem. 39(8):1682-1685, 1993.
Fiore et al, (1988), "The Abbott IMx automated benchtop immunochemistry analyzer system", Clin. Chem. 34(9):1726-2732.
Matthews et al, (1988), "Analytical strategies for the use of DNA probes", Anal. Biochem. 169:1-25.
Southern et al, (1992), "Analyzing and comparing nucleic acid sequences by hybridization to arrays of oligonucleotides: Evaluation using experimental models", Genomics 13:1008-1017.
Stratagene Catalog, (1993) p. 105.
Weisberg et al, (1993), "Simultaneous mutagenesis of multiple sites: Application of ligation chain reaction using PCR products instead of oligonucleotides", Biotechniques 15(1):68-74.
Cotton et al, (1993), "Current Methods of mutation detection", Mutation Res. 285:135-144.
Abravaya et al, (1995), "Detection of point mutations with a modified ligase chain reaction (Gap-LCR)", Nucleic Acids Res. 23(4):675-682.
D.J. Picketts et al, Human Genetics, "Differential Termination of Printer Extension: A Novel, Quantifiable Method for Detection of Point Mutations", vol. 89, pp. 155-157 (1992).
E. Winn-Deen et al, Poster Presentation-ASHG Annual Meeting, "High Density Multiplex Mutation Analysis Using the Oligonucleotide Ligation Assay (OLA) and Sequence-Coded Separation", Abstract #1512, pp. 1-4 (1993).
M. Goldrick Ph.D., Ambion TechNotes, "Faster Mutation Analysis", vol. 2, No. 1, pp. 1 and 10.
R. Youil et al, Proc. Natl. Acad. Sci. USA, "Screening for Mutations by Enzyme Mismatch Cleavage with T4 Endonuclease VII", vol. 92, pp. 87-91 (1995).
A-Lien Lu et al, Genomics 14, "Detection of Singe DNA Base Mutations with Mismatch Repair Enzymes", pp. 249-255 (1992).
E.M. Southern et al, Genomics 13, "Analyzing and Comparing Nucleic Acid Sequences by Hybridization to Arrays of Oligonucleotides: Evaluation Using Experimental Models", pp. 1008-1017 (1992).
A. Pease et al, Proc. Natl. Acad. Sci. USA, "Light-Generated Oligonucleotide Arrays for Rapid DNA Sequence Analysis", vol. 91, pp. 5022-5026 (1994).
J. Dianzani et al, TIG, "Dilemmas and Progress in Mutation Detection", vol. 9, No. 12, pp. 1-3, (1993).
S. Borman, C&EN, Science/Technology, "`DNA Chips` Under Development for Sequencing Applications", pp. 24-25 (1994).
N. Dolinnaya et al, Nucleic Acids Research, "Oligonucleotide Circularization By Template-Directed Chemical Ligation", vol. 21, No. 23, pp. 5403-5407 (1993).
N. Dolinnaya et al, Nucleic Acids Research, "Structural and Kinetic Aspects of Chemical Reactions in DNA Duplexes. Information on DNA Local Structure Obtained From Chemical Ligation Data", vol. 19, No. 11, pp. 3073-3080 (1991).
N. Dolinnaya et al, FEBS 09843, "Probing DNA Triple Helix Structure by Chemical Ligation", vol. 284, No. 2, pp. 232-34 (1991).
N. Dolinnaya et al, Nucleic Acids Research, "The Use of BrCN for Assembling Modified DNA Duplexes and DNA-RNA Hybrids; Comparison with Water-Soluble Carbodiimide", vol. 19, No. 11, pp. 3067-3072 (1991).
S. Nilsson et al, Nucleic Acids Research, "Sealing of Gaps in Duplex DNA T4 DNA Ligase", vol. 10, No. 5, (7 pages included, no page numbers) (1982).
Bhatnagar Satish K.
George, Jr. Albert L.
Nazarenko Irina
Fredman Jeffrey
Jones W. Gary
Karta Glenn E.
Oncor, Inc.
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