Method for amplifying unknown nucleic acid sequences

Details Method for amplifying unknown nucleic acid sequences Method for amplifying unknown nucleic acid sequences

1989-12-21

1992-04-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 27, 935 77, 935 78, C12Q 168, C07H 1512, C12N 1500

Patent

active

051047926

ABSTRACT:
A modification of the PCR technique is described which allows fragments of RNA or DNA to be amplified without prior knowledge of their sequence. The technique can be used to amplify viral nucleic acids present in small amounts in clinical material allowing, for example, the diagnosis of a particular virus infection or the discovery of new viruses.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Sanger et al, J. Mol. Biol., vol. 143, 198, pp. 161-178.

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