Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-01-25
2005-01-25
Sisson, Bradley L. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100, C536S024300, C536S024330, C536S025300
Reexamination Certificate
active
06846626
ABSTRACT:
Disclosed is a method of specifically amplifying desired regions of nucleic acid from a sample containing nucleic acid. The method includes providing a plurality of first PCR primers, each first primer having a region of fixed nucleotide sequence identical or complementary to a consensus sequence of interest and a region of randomized nucleotide sequence located 5′ to, 3′ to, anywhere within, or flanking the region of fixed nucleotide sequence; providing a plurality of second PCR primers, each second primer having a region of arbitrary, yet fixed nucleotide sequence and a region of randomized nucleotide sequence located 5′ to, 3′ to, anywhere within, or flanking the region of fixed nucleotide sequence; and then amplifying the nucleic acid present in the sample via the PCR using the plurality of first PCR primers and the plurality of second PCR primers; whereby a subset of the plurality first primers binds to the consensus sequence of interest substantially wherever it occurs in the sample, and a subset of the plurality of second primers binds to the sample at locations removed from the first primers such that DNA regions flanked by the first primer and the second primer are specifically amplified.
REFERENCES:
patent: 5200313 (1993-04-01), Carrico
patent: 5508169 (1996-04-01), Deugau et al.
patent: 5807679 (1998-09-01), Kamb
patent: 6521428 (2003-02-01), Senapathy
patent: 6528288 (2003-03-01), Senapathy
Sommer and Tautz, “Minimal homology requirements for PCR,” Nucleic Acids Research, vol. 17, No. 16 (1989), p. 6749.*
Bowie, et al.,Science,247:1306 (1990)).
Deeb, S.S., et al.,Am. J. Hum. Genet.,46:822 (1990).
Duyk, G.M., et al.,Proc. Natl. Acad. Sci. USA,87:8995-9 (1990).
Estivill, X. and Williamson, R.,Nucleic Acids Res.,15:1415-25 (1987).
Fuentes, J.J., et al.,Hum. Genet.101:346-50 (1997.
Jendraschak, E. and Kaminski, W.E.,Genomics,50:53-60 (1998).
Lim, J., et al.,Mol. Cell. Endocrinol.,131:205 (1997).
Lovett, M., et al.,Proc. Natl. Acad. Sci. USA,88:9628-32 (1991).
Myerowitz, T.,Proc. Natl. Acad. Sci. USA,85:3955 (1988).
O Neill, M.J., et al.,Hum. Mutat.,11:340 (1998).
Reissner, K., et al.,Mol. Genet. Metab.,63:281 (1998).
Rommens, et al.,Science,245:1059-80 (1989).
Senapathy, P.,Proc. Natl. Acad. Sci.,83:2133-2137 (1986).
Senapathy, P. et al., RNA splice junctions of different classes of eukaryotes: Sequence statistics and functional implications in gene expression, Nucleic Acids Research, vol. 15, No. 17, pp. 7155-7176 (1987).
Senapathy, P.,Proc. Natl. Acad. Sci.,85:1129-1133 (1988).
Senapathy, P.,Molecular Genetics(Life Sci. Adv.), 7:53-65 (1988).
Senapathy, P., et al., 1990. Splice junctions, branch point sites, and exons: Sequence statistics, identification, and applications to the Genome Project, inMethods in Enzymology, Computer Analysis of Protein and Nucleic Acid Sequences,Doolittle, R.F., ed., 183:252-278.
Tajima, T., et al.,Endoc. J.,45:291 (1998).
Wilson, R., et al.,Nature,368:32-38 (1994).
DeWitt Ross & Stevens S.C.
Genome Technologies LLC
Leone, Esq. Joseph T.
Sisson Bradley L.
LandOfFree
Method for amplifying sequences from unknown DNA does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for amplifying sequences from unknown DNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for amplifying sequences from unknown DNA will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3434077