Method for amplifying nucleic acid sequence

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S091100, C435S091200, C536S022100, C536S023100, C536S024300, C536S024310, C536S024320, C536S024330

Reexamination Certificate

active

06951722

ABSTRACT:
A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.

REFERENCES:
patent: 5648211 (1997-07-01), Fraiser et al.
patent: 5824517 (1998-10-01), Cleuziat et al.
patent: 6251639 (2001-06-01), Kurn
patent: 0 496 483 (1992-07-01), None
patent: 05/308963 (1993-11-01), None
patent: WO 96/19572 (1996-06-01), None
patent: WO 96/40901 (1996-12-01), None
patent: WO 99/09211 (1999-02-01), None
US 5,711,311, 4/1998, Fraiser et al. (withdrawn)
Sambrook, et al., “Molecular Cloning: A Laboratory Manual, Third Edition”, 2001. vol. 3, A4.15, A4.16 and A4.38.
Burrows, et al., “Purification and properties of DNA polymerase fromBacillus caldotenax”, Biochem Journal. Nov. 1, 1992, vol. 287 (Pt 3), 971-977.
Itaya, M., “Isolation and characterization of a second RNase H (RNase Hll) ofEscherichia coliK-12 encoded by thernhBgene”, Proc. Natl. Acad. Sci. Nov. 1990, vol. 87, 8587-8591.

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