Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-03-20
1999-11-09
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 9121, 435 913, 435 915, 435 9151, 435 911, 536 243, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104
Patent
active
059811838
ABSTRACT:
In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 5130238 (1992-07-01), Malek et al.
patent: 5268289 (1993-12-01), Dahl et al.
patent: 5322770 (1994-06-01), Gelfand
patent: 5407800 (1995-04-01), Gelfand et al.
patent: 5409818 (1995-04-01), Davey et al.
patent: 5459055 (1995-10-01), Jendrisak et al.
Date et al. (1975) J. Biochem. 78:845-58.
Kanaya et al. (1992) J. Biol. Chem. 267:10184-92.
Fahy et al. (1991) PCR Methods Appl. 1:25-33.
Fahy et al. (1994) PCR Methods Appl. 3:S83-S94.
Gingeras et al. (1991) J. Infect. Disease 164:1066-74.
Guatelli et al. (1990) Proc. Natl. Acad. Sci. USA 87:1874-78.
Kievits et al. (1991) J. Virol. Methods 35:273-86.
Zehbe et al. (1994) Cell Viosion 1:46-47.
Zehbe et al. (1994) Cell Vision 1:20-24.
Zehbe et al. (1995) Cell Vision 2:240-2.
Sooknannan et al. (1995) Bio/Technology 13:563-564.
Walter et al. (1994) Proc. Natl. Acad. Sci. USA 91:7937-41.
Vandamme et al. (1995 March) J. Virol. Methods 52:121-32.
The Journal of Biological Chemistry vol. 267, No. 14, May 15, 1992 S. Kanaya "Expression Purification and Characterization of a Recombinaut Ribonuclease H from Thermus thremophilus HB8" pp. 10184-10192.
Inoue Hiroaki
Kawamura Yoshihisa
Shibata Shuji
Takarada Yutaka
Horlick Kenneth R.
Toyo Boseki Kabushiki Kaisha
Tung Joyce
LandOfFree
Method for amplifying and detecting of target nucleic acid seque does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for amplifying and detecting of target nucleic acid seque, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for amplifying and detecting of target nucleic acid seque will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1453916