Method for amplifying a polynucleotide

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 435 9152, C12Q 168, C12P 1934

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active

059142294

ABSTRACT:
In one aspect, the present invention relates to methods of amplifying a target nucleic acid, the methods involving the production of a double-stranded promoter to provide for transcription of the target sequence, and the provision of a poly-A tail in the transcription product. In another aspect, the present invention relates to methods of detecting a target nucleic acid in a sample.

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