Method for amplification of nucleotide sequence

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S024330

Reexamination Certificate

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07977055

ABSTRACT:
To provide a method for selectively amplifying a target nucleic acid and a method for detecting a nucleic acid by the method, which are useful as a method for synthesizing the nucleic acid. A method for amplifying a nucleic acid sequence (EVA (Endonuclease V-dependent Amplification) method) which selectively amplifies a target nucleic acid in a sample, by the use of an oligonucleotide primer containing a base which can be recognized by an endonuclease V, the endonuclease V and a DNA polymerase having a strand displacement activity, and a method for detecting a nucleic acid by the method.

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Turner, D. J. et al. Harnessing asymmetrical substrate recognition by thermostable EndoV to achieve balanced linear amplification in multiplexed SNP typing. Biochem.Cell Biol., vol. 84, pp. 232-242, Apr. 2006.
Ehses, S., et al., “Optimization and design of oligonucleotide setup for strand displacement amplification”, Journal of biochemical and biophysical methods, 2005, pp. 170-186, XP 004963640.
European Official Communication, dated Apr. 8, 2011, issued in Application No. 07 767 254.1.

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