Method for amplification of nucleic acids in solid media

Details Method for amplification of nucleic acids in solid media Method for amplification of nucleic acids in solid media

1992-10-26

1997-04-01

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, C12P 1934, C12Q 160

Patent

active

056164785

ABSTRACT:
Amplification and/or expression of nucleic acids is carried out in a medium immobilized by using an organic and/or inorganic solid matrix penetrating the medium and having a porous, fibrous, reticulated, coiled, capillary, lamellar or folded texture and which includes the components of a cell-free enzyme system of exponential amplification of nucleic acids and/or components of a cell-free enzyme system of nucleic acid expression. In this medium, the progeny of each molecule (clone) and the expression products remain in the same zone of the reaction volume where the matrix molecule was initially located. The method permits cloning of nucleic acids in vitro as well as detection of solitary nuleic acid molecules in the sample studied.

REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5004543 (1991-04-01), Pluskal et al.
patent: 5188963 (1993-02-01), Stapleton
patent: 5190864 (1993-03-01), Giese et al.
patent: 5382511 (1995-01-01), Stapleton et al.
Maniatis et al, Molecular Cloning (1982) p. 161.
Chetverin et al., J. Mol. Biol 222:3-9 (1991) "On the Nature of Spontaneous RNA . . .".
Guatelli et al., P.N.A.S.. 87:1874-1878 (1990) Isothermal, in vitro amplification of nucleic acids.
Erlich, H. A. et al. "Recent Advances . . . " Science (1991) 252, pp. 1643-1651.
Guatelli, J. C. et al. "Isothermal" . . . Proc. Natl'l Acad. Sci. (1990) 87, pp. 1874-1878.
Haruna, I. et al., "Autocatalytic Synthesis . . ." Science 150 (1965) pp. 884-886.
Kamen, R. I. "Structure and Function . . . " In RNA Phages (1975) pp. 203-234.
Levisohn, R. et al. "The Cloning of a . . . " Proc. Natl'l Acad. Sci. U.S.A. 60, pp. 866-872.
Lizardi, P. M. et al. "Exponential Amplification . . . " Bio/Technology 6, pp. 1197-1202.
Lomell, H. et al. "Quantitative Assays . . . " Clin. Chem. 35, (1989) pp. 1826-1831.
Miele, A. A. et al. "Autocatalytic Replication . . . " J. Mol. Biol. (1983) 171, pp. 281-295.
Mullis, K. B. et al. "Specific Synthesis of DNA . . . " Methods Enzymol. (1987) 155, pp. 335-350.
Munishkin, A. V. et al. "Efficient Templates . . . " J. Mol. Biol. (1991) 221, pp. 463-472.
Myers, T. W. et al. "Reverse Transcription . . . " Biochemistry 30, (1991) pp. 7661-7666.
Saiki, R. K. et al. "Enzymatic Amplification . . . " Science 230, (1985), pp. 1350-1354.
Sumper, M. et al. "Evidence for de Novo . . . " Proc. Nat'l. Acad. Sci. U.S.A. (1975) pp. 162-166.
Wu, Y. et al. "Amplifiable messenger RNA" Proc. Nat'l. Acad. Sci. USA (1992).
Anderson, C.W. et al. "Preparation of a Cell-Free . . . " Methods Enzymol. (1983) 101, pp. 635-644.
Baranov, V. I. et al. "Gene Expression . . . " Gene 84 (1989) pp. 463-466.
Bujard, H. et al. "A T5 Promoter-based . . . " Methods Enzymol. 155 (1987) pp. 416-433.
Chambliss, G. H. et al. "Bacterial in vitro . . . " Methods Enzymol. 101, (1983) pp. 598-605.
Chen, H.-Z. et al. "Prokaryotic Coupled . . . " Methods Enzymol. 101, (1983) pp. 674-690.
Lesley, S. A. et al. "Use of in vitro . . . " J. Biol. Chem. 266 (1991) pp. 2632-2638.
Merrick, W.C. "Translation of Exogenous . . . " Methods Enzymol. 101 (1983) pp. 606-615.
Tymms, M. J. et al. "Efficient in vitro . . . " Gene, Anal. Tech. 5, (1988) pp. 9-15.
Chetverin, Alexander B. et al. "On the Nature of Spontaneous RNA Synthesis by QB Replicase", J. Mol. Biol. (1991) 222, pp. 3-9.

Affiliated with

Also associated with

Rating

Method for amplification of nucleic acids in solid media does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for amplification of nucleic acids in solid media, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for amplification of nucleic acids in solid media will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-538206