Method for amplification and detection of RNA and DNA sequences

Details Method for amplification and detection of RNA and DNA sequences Method for amplification and detection of RNA and DNA sequences

1994-11-03

1997-04-22

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 935 77, 935 78, C12Q 170, C12Q 168, C12P 1934

Patent

active

056228201

ABSTRACT:
A method for detecting false negative and false positive results in a method of detecting and identifying specific human nucleic acid sequences contained in human nucleic acids in a human blood or tissue sample, comprising: (1) amplifying at least one portion of the specific nucleic acid sequence present in the sample and simultaneously simplifying a marker sequence; (2) transcribing the amplification products with an RNA polymerase to produce multiple RNA copies of each copy of specific nucleic acid sequence comprising the amplification product; and (3) identifying the transcription products.

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