Method for amino acid sequence analysis

Details Method for amino acid sequence analysis Method for amino acid sequence analysis

1992-02-26

1993-08-10

Johnston, Jill A.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436161, 436172, G01N 3368

Patent

active

052348369

ABSTRACT:
The present invention is directed to a method for amino acid sequence analysis comprising either the steps of labeling the sample with a fluorescent reagent and quenching the excess fluorescent reagent remaining after said labeling with an ammonium salt, or the steps of degrading amino acid from the amino terminus of peptides or proteins using a fluorescent Edman reagent and quenching the excess fluorescent Edman reagent remaining after said degrading with an ammonium salt. The method of the present invention makes it possible to eliminate the interference of identification by the chromatographic peak of fluorescent reagent by quenching the excess fluorescent reagent in the sample.

REFERENCES:
patent: 4865994 (1989-09-01), Tsugita et al.
Kent, S., et al, "Approaches to Sub-Picomole Protein Sequencing", vol. 5, No. 4, (1987), BioTechniques, pp. 314-320.
Database WPIL, week 8648, Derwent Publications, Ltd., London, GB; AN 86-315348 & JP-A-61 233371 (Toyo Soda Mfg KK) 17 Oct. 1986.
Database WPIL, Week 8642, Derwent Publications, Ltd., London GB; AN 86-275161 & JP-A-61 199983 (Fuji Photo Film KK) 4 Sep. 1986.

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