Method for altering a nucleotide sequence

Details Method for altering a nucleotide sequence Method for altering a nucleotide sequence

1993-03-29

1994-08-02

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 9141, 435 9151, 4351723, 435227, C07H 2104, C12N 1510, C12N 1511, C12P 1934

Patent

active

053345150

ABSTRACT:
A method and kit, employing exo-sample nucleotides such as deoxyuridine, capable of altering the nucleic acid sequence present at the 3' or 5' end of a DNA or RNA molecule is provided. The method and kit can be used to achieve the selective amplification of nucleic acid molecules.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4873192 (1989-10-01), Kunkel
patent: 5130238 (1992-07-01), Malek et al.
Duncan, B., The Enzymes XIV:565-586 (1981).
Longo, M. C. et al., Gene 93:125-128 (1990).
Frohman, M. A. et al., Proc. Natl. Acad. Sci. (U.S.A.) 85:8998-9002 (1988).
Ohara, O. et al., Proc. Natl. Acad. Sci. (U.S.A.) 86:5673-5677 (1989).
Sambrook, J. et al. Molecular Cloning: A Laboratory Manual, 2nd ed. (1989) p. 14.6.

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