Method for albumin purification

Details Method for albumin purification Method for albumin purification

2008-09-09

2008-09-09

Mondesi, Robert B. (Department: 1652)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Albumin

Reexamination Certificate

active

10514536

ABSTRACT:
The present invention is a method of purifying recombinant human serum albumin (rHSA) from a solution, which method comprises to subject a cell culture supernatant (CCS) comprising rHSA cation exchange on a bimodal high salt tolerant matrix; hydrophobic interaction chromatography (HIC); anion exchange; and recovering the purified rHSA. The bimodal high salt tolerant cation exchange matrix used enables performing a purification of a cell culture supernatant directly, in the sense that no further dilution thereof is necessary. Due to its high binding capacity, said bimodal cation exchange matrix also allows use of a smaller amount of matrix as compared to a corresponding conventional cation exchanger matrix. Accordingly, the present invention allows substantial savings as regards volumes and consequently operation costs.

REFERENCES:
patent: 5440018 (1995-08-01), Ohmura et al.
patent: 0 570 916 (1993-05-01), None
patent: 0 612 761 (1994-02-01), None
patent: WO 02/05959 (2002-01-01), None

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