Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1981-06-22
1988-02-02
Tarcza, J. E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351722, 435240, 935108, 530413, 436548, C12P 2100, C12N 1500, C12N 500
Patent
active
047228962
ABSTRACT:
An efficient process design for purifying large quantities of specific antibodies by affinity chromatography is disclosed. By utilizing the process strategy described, large scale production of highly purified hybridoma antibodies and other proteins becomes feasible.
The initiation of the process centers on production of hybridoma proteins which bind to a commonly available inexpensive protein such as human serum albumin (HSA) with which large quantities of a protein such as mouse immunoglobulin can be isolated by affinity chromatography. The mouse immunoglobulin is covalently linked to an inert matrix, such as Sepharose beads and this affinity reagent is used to purify a large quantity of a hydridoma protein such as rat anti-mouse IgG antibody. This rat anti-mouse IgG antibody can then be linked to Sepharose beads and can be used as a general affinity reagent for the purification of any mouse immunoglobulin.
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Tarcza J. E.
The Beth Israel Hospital Association
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