Method for adsorption and elution of lipid hydrolyzing enzymes

Details Method for adsorption and elution of lipid hydrolyzing enzymes Method for adsorption and elution of lipid hydrolyzing enzymes

1974-09-18

1977-03-22

Monacell, A. Louis

Textiles: fiber preparation

195 63, C07G 702

Patent

active

040135128

ABSTRACT:
A lipid hydrolyzing enzyme-containing substance is brought into contact with an insoluble carrier having a hydrophobic organic compound attached thereto in the form of a hydrophobic residue so that the lipid hydrolyzing enzyme is adsorbed by the hydrophobic residue attached to the insoluble carrier. Then, the lipid hydrolyzing enzyme adsorbed on the carrier is isolated by elution.

REFERENCES:
patent: 3649455 (1972-03-01), Abe et al.
Er-el et al., "Hydrocarbon-Coated Sepharoses. Use in the Purification of Glycogen Phosphorylase," Biochemical & Biophysical Research Communications 49:2 (1972), pp. 383-390.
Hofstee, "Protein Binding by Agarose Carrying Hydrophobic Groups," Biochemical & Biophysical Research Communications 50:3 (1973), pp. 751-757.
Advances in Enzymology, vol. 36, pp. 29-89, pp. 29 and 46-50, 1972.

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