Method for adhering fecal samples to slides

Chemistry: analytical and immunological testing – Including sample preparation – Stabilizing or preserving

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436 17, G01N 128

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active

054533813

ABSTRACT:
This invention provides methods and compositions for the analysis of parasites in fecal samples. A parasitological fixative is provided which contains glyceraldehyde, a salt of an organic acid, organic solvent and water. The parasitological fixative does not contain formaldehyde, formalin or other harmful chemicals commonly associated with fixative compositions. The invention further describes a slide fixative composition comprising a protein solution, glycerin and an adhesion enhancing agent which improves the adhesion of sample components to a microscope slide.

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Burrows, "A New Fixative and Technics for the Diagnosis of Intestinal Parasites," Technical Bulletin of the Registry of Medical Technologies, vol. 37, No. 8, pp. 208-212 (1967).
Brooke, "PVA-Fixative Technique in the Laboratory Confirmation of Amoebiasis," Triangle 4: 326-335 (1960).
Hague et al. "Clinical Comparison of a Formaldehyde Free Histological Fixation to Neutral Buffered Formalin," Abstract submitted to the International Academy of Pathology, Sep. 1992.
Meridian Diagnostics, Inc., Product Bulletin for Para-Pak.TM. (SAF Fixative), 1979.
NoTox.RTM. Product Brochure.
Sapero et al. "The `MIF` Stain-Preservation Technic for the Identification of Intestinal Protozoa," Am. J. Trop. Med. Hyg. 2:613-619 (1953).

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