Method for activation of transient receptor potential cation...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S325000, C436S501000

Reexamination Certificate

active

07960130

ABSTRACT:
The present invention relates to a method for activation of TRPA1 (transient receptor potential cation channel, subfamily A, member 1) using acetaldehyde, more precisely a method for selecting a candidate for TRPA1 activation blocker from neurons activated by acetaldehyde. Acetaldehyde of the present invention works on TRPA1 specifically so that it facilitates the isolation of sensory neurons expressing TRPA1. Therefore, acetaldehyde of the invention can be effectively used for the studies on TRPA1 mechanisms and the development of a TRPA1 based anodyne.

REFERENCES:
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Jordt SE et al. Mustard oils and cannabinoids excite sensory nerve fibers through the TRP channel ANKTM1. Nature, 2004; 427(6971):260-265.
Story GM et al. ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell, Mar. 21, 2003; 112(6):819-829.
Bradley DM et al. In vitro comparison of the effects of ethanol and acetaldehyde on dorsal root ganglion neurons. Alcohol Clin Exp Res. 1995; 19(5):1345-1350.
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Bang S. et al., (Apr. 26, 2007). Transient receptor potential A1 mediates acetaldehyde-evoked pain sensation. The 15th Meeting of Korean Basic Medical Scientists.
Bang S. et al., (Oct. 23, 2007). Transient receptor potential A1 mediates acetaldehyde-evoked pain sensation. Eur. J. Neurosci. 26(9): 2516-23.
Namer B. et al., (Jun. 2005). TRPA1 and TRPM8 activation in humans: effects of cinnamaldehyde and menthol. Neuroreport. 16(9):955-959.
Trevisani M. et al., (Aug. 7, 2007). 4-Hydroxynonenal, an endogenous aldehyde, causes pain and neurogenic inflamation through activation of the irritant receptor TRPA1. Proc. Natl. Acad. Sci. U.S.A. 104(33):13519-13524.

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