Method for activating prethrombin-1

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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Reexamination Certificate

active

08062876

ABSTRACT:
Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

REFERENCES:
patent: 03/070746 (2003-08-01), None
Rao et al.,Blood102(4):13471354, 2003.
Joseph et al.,Blood94(2):621-631, 1999.
Pierre et al.,Mol. Biol. Evol.22(9):1853-1864, 2005.
Welton et al.,Toxicon46:328-336, 2005.
Rao et al.,Thromb Haemost88:611-619, 2002.
Rao et al.,Thromb Haemost92:509-521, 2004.
Kini,Toxicon45:1133-1145, 2005.
Speijer et al.,J. Biol. Chem.261(28):13258-13267, 1986.
Takashi Morita et al., “Prothrombin Activator fromEchis carinatusVenom”; Method in Enzymology, vol. 80, pp. 303-311.
Ramona J. Petrovan et al., Purification and Characterization of Multisquamase, the Prothrombin Activator Present inEchis multisquamatusVenom; Thrombosis Research 88 (1997), pp. 309-316.
Australian Office Action mailed May 13, 2009.

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