Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-09-23
2000-04-18
Fox, David T.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4353201, 435419, 536 2372, 800205, 800DIG17, 800DIG26, 800DIG27, 800DIG43, 800DIG56, 800DIG57, 800DIG58, 800DIG63, A01H 500, C12N 514, C12N 1533, C12N 1582
Patent
active
060514094
ABSTRACT:
The present invention provides an improved method for achieving stable integration of an exogenous DNA fragment in intact form into the genome of a eukaryotic cell, particularly a plant cell. The method comprises providing the exogenous DNA together with one or more proteins which promote integration of the exogenous DNA to the eukaryotic cell targeted for transformation, wherein the proteins are provided in the form of a chimeric gene or translatable RNA capable of expression in the eukaryotic cell. The method is particularly applied to plant cells to achieve stable integration of an exogenous DNA fragment bounded by T-DNA borders in intact form using integration-promoting proteins derived from Agrobacterium. Transgenic cultures, tissues and whole organisms, particularly transgenic plants, can be generated from cells transformed according to the method of the invention.
REFERENCES:
patent: 5036006 (1991-07-01), Sanford et al.
patent: 5231019 (1993-07-01), Paszkowski et al.
patent: 5302523 (1994-04-01), Coffee et al.
Citovsky, et al., "Nuclear Localization of Agrobacterium VirE2 Protein in Plant Cells", Science, 256:1802-1805 (1992).
Escudero, et al., "Intracellular Agrobacterium can transfer DNA to the cell nucleus of the host plant", Proc. Natl. Acad. Sci., 92:230-234 (1995).
Tinland, et al. "The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integratio of T-DNA into the plant genome", The EMBO Journal, 14(14):3585-3595 (1995).
Zupan, et al., "Transfer of T-DNA from Agrobacteriumto the Plant Cell", Plant Physiol., 107:1041-1047 (1995).
Chilton, Mary-Dell, "Agrobacteriumgene transfer: Progress on a poor man's vector for maize", Proc. Natl. Acad. Sci. USA, 90:3119-3120 (1993).
Citovsky, et a., "Nuclear import of AgrobacteriumVirD2 and VirE2 proteins in maize and tobacco", Proc. Natl. Acad. Sci. USA, 91:3210-3214 (1994).
De Vos, et al., "Expression of AgrobacteriumNopaline-Specific VirD1, VirD2, and VirC1 Proteins ant Their Requirements for T-strand Production E. coli", Molecular Plant-Microbe Interactions, 2(2):43-52 (1989).
Gallie, et al., "Visualizing mRNA Expression in Plant Protoplasts: Factors Influencing Efficient mRNA Uptake and Translation", The Plant Cell, 1:301-311 (1989).
Gallie, Daniel R., "Introduction of mRNA to plant protoplasts using polyethylene glycol", Plant Cell Reports, 13:119-122 (1993).
Ghai, et al., "The virD operon of Agrobacterium tumefaciens Ti plasmid encodes a DNA-relaxing enzyme", Proc. Natl. Acad. Sci. USA, 86:3109-3113 (1989).
Gheysen, et al., "Illegitimate recombination in plants: a model for T-DNA integration", Genes & Development, 5:287-297 (1991).
Herman, et al., "Plant chromosome/marker gene fusion assay for study of normal and trncated T-DNA integration events", Mol. Gen. Genet., 224:248-256 (1990).
Jasper, et al., "AgrobacteriumT-strand production in vitro: Sequence-specific cleavage and 5' protection of single-stranded DNA templates by purified VirD2 protein", Proc. Natl. Acad. Sci. USA, 91:694-698 (1994).
Klein, et al., "Stable genetic transformation of intact Nicotianacells by the particle bombardment process", Proc. Natl. Acad. Sci. USA, 85:8502-8505 (1988).
Koncz, et al., "9. Homology Recognition During T-DNA Integration into the Plant Genome", J. Paszkowski (ed.), Homologous Recombination and Gene Silencing in Plants, 167-189 (1994).
Koziel, et al., "Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis", Bio/Technology, 11: 194-200 (1993).
Matzke, et al., "How and Why Do Plants Inactive Homologous (Trans)genes?.sup.1 ", Plant Physiol., 107:679-685 (1995).
Mayerhofer, et al., "T-DNA integration: a mode of illegitimate recombination in plants", The EMBO Journal, 10(3):697-704 (1991).
Negrutiu, et al., "Hybrid genes in the analysis of transformation conditions", Plant Molecular Biology, 8:363-373 (1987).
Ohba, et al., "DNA rearrangement associated with the integration of T-DNA in tobacco: an example for multiple duplications of DNA around the integration target", The Plant Journal, 7(1):157-164 (1995).
Okada, et al., "Introduction of Functional RNA into Plant Protoplasts by Electroporation", Plant Cell Physiol., 27(4):619-626 (1986).
Pansegrau, et al., "Site-specific cleavage and joining of single-stranded DNA by VirD2 protein of Agrobacterium tumefaciens Ti plasmids: Analogy to bacterial conjugation", Proc. Natl. Acad. Sci. USA, 90:11538-11542 (1993).
Rasmussen, et al., "Biolistic transformation of tobacco and maize suspension cells using bacterial cells as microprojectiles", Plant Cell Reports, 13:212-217 (1994).
Regensburg-Tuink, et al., "Transgenic N. glauca plants expressing bacterial virulence gene virFare converted into hosts for nopaline strains of A. tumefaciens", Nature, 363:69-71 (1993).
Rossi, et al., "The VirD2 protein of Agrobacterium tumefaciens carries nuclear localizatino signals important for transfer of T-DNA to plants", Mol. Gen. Genet., 239: 345-353 (1993).
Saul, et al., "Direct DNA transfer to protoplasts with and without electroporation", Plant Molecular Biology Manual, AI:1-16 (1988).
Stachel, et al., "Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells", Nature, 322:706-712 (1986).
Tinland, et al., "The Agrobacterium tumefaciens Virulence D2 Protein is Responsible for Precise Integration of T-DNA into the Plant Genome", Friedrich Miescher-Institut, 1-39.
van Haaren, et al., "Mutational analysis of the conserved domains of a T-region border repeat of Agrobacterium tumefaciens", Plant Molecular Biology, 13:523-531 (1989).
Vogel, et al., "Mutational Analysis of Agrobacterium tumefaciens virD2: Tyrosine 29 Is Essential for Endonuclease Activity", Journal of Bacteriology, 174(1):303-308 (1992).
Walker, et al., "GUS mRNA Delivery and Expression in Plant Cells via Particle Bombardment", Annual Meeting Abstracts, Abstract #218.
Chilton Mary-Dell
Hansen Genevieve
Fox David T.
Hoxie, Esq. Thomas
Nelson Amy J.
Novartis Finance Corporation
LandOfFree
Method for achieving integration of exogenous DNA delivered by n does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for achieving integration of exogenous DNA delivered by n, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for achieving integration of exogenous DNA delivered by n will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2335472