Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-05-17
2009-12-01
Gabel, Gailene R (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007230, C435S007240, C435S285200, C435S287100, C435S287200, C435S288700, C436S010000, C436S017000, C436S056000, C436S164000, C436S171000, C436S175000, C436S546000, C422S082010, C422S082050, C422S082070
Reexamination Certificate
active
07625712
ABSTRACT:
A method useful for the enumeration of cell populations in a biological sample includes the steps of reacting in a single reaction mixture a sample, a first antibody labeled with a fluorochrome having a first emission spectrum and an additional antibody. The first antibody binds to an antigenic determinant differentially expressed on leukocytes and non-leukocytes. The additional antibody binds to an antigenic determinant differentially expressed on mature and immature granulocytes or myeloid cells, and is labeled either with the first fluorochrome or an additional fluorochrome having an emission spectrum distinguishable from the first emission spectrum. The reaction mixture can be mixed with a nucleic acid dye having an emission spectrum that overlaps with one of the first or additional emission spectra. The reaction mixture may be treated with a lytic system that differentially lyses non-nucleated red blood cells and conserves leukocytes. Populations of hematological cells are detected and enumerated using at least two parameters (fluorescence, optical, and electrical) for each population.
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Careaga Diana B.
Carrillo Barbara
Lopez Lidice L.
Paul Ronald D.
Rubio Oilda
Alter, Esq. Mitchell E.
Beckman Coulter Inc.
Gabel Gailene R
Howson & Howson LLP
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