Method, composition and kit for isoelectric focusing

Details Method, composition and kit for isoelectric focusing Method, composition and kit for isoelectric focusing

2011-05-17

2011-05-17

Ruddock, Ula C (Department: 1759)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S644000

Reexamination Certificate

active

07943029

ABSTRACT:
The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra.

REFERENCES:
patent: WO2005/103070 (2005-11-01), None
Johnsson, B., et al. “Comparison of methods for immobilization to carboxymethyl dextran sensor surfaces by analysis of the specific activity of monoclonal antibodies” Journal of Molecular Recognition, vol. 8, 1995, p. 125-131.
Gianazza, E., et al. “Formulations for immobilized pH gradients including pH extremes” Electrophoresis, vol. 10, 1989, p. 806-808.
Bier, M., et al., “Recycling isoelectric focusing: use of simple buffers” Journal of Chromatography, 604 (1992) 73-83.
Frey, M., et al., “High Resolution preparative isoelectric focusing in layers of granulated gels” Electrophoresis, 3 (1982) 216-266.
Yang, C., et al., “Repeatedly usable immobilized pH gradient in a monolithic capillary column” Electrophoresis, 25 (2004) 1729-1734.

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