Method, composition and element for the detection of nitrogen-co

Details Method, composition and element for the detection of nitrogen-co Method, composition and element for the detection of nitrogen-co

1978-02-24

1979-11-27

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

23230B, 23232R, 435 39, G01N 3316

Patent

active

041760085

ABSTRACT:
Methods, compositions and elements for detecting a nitrogen-containing compound capable of releasing ammonia upon enzymatic action are described. The method comprises contacting in an aqueous medium a sample suspected of containing such a compound and a novel assay composition comprising at least one enzyme which catalyzes the decomposition of the compound to ammonia and detecting the ammonia by condensation with a diketone and measuring the color or fluorescence produced by the resulting dihydropyridine.
The foregoing assay composition can be incorporated into single-layer or multilayer analytical elements of the type known in the prior art. A preferred such element comprises an isotropically porous spreading layer in fluid contact with a reagent layer. The spreading layer is most preferably non-fibrous.

REFERENCES:
patent: Re27524 (1972-11-01), Nadeau
patent: 3145086 (1964-08-01), Free
patent: 3806416 (1974-04-01), Mollering et al.
patent: 3873269 (1975-03-01), Kraffczyk et al.
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4125377 (1978-11-01), Gindler
Hantzsch, Ann. Chem. Liebigs, 215, p. 1, 1882 (translation of pp. 5-8, only).
Knoevenagel, Annalen, 281, p. 25, 1894 (translation of pp. 25-28, only).
Dunsbach in Chemical Abstracts, vol. 66 (1967), 82993a.

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