Method, composition and device for the determination of choleste

Details Method, composition and device for the determination of choleste Method, composition and device for the determination of choleste

1991-03-05

1996-01-30

Gitomer, Ralph J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 19, 435 28, 435190, 435805, C12Q 160, C12Q 128, C12N 904

Patent

active

054879787

ABSTRACT:
The present invention provides a kinetic or rate method for the determination of cholesterol in free or bound form with of a cholesterol oxidase enzyme obtained from Bacterial strain NRRL B-18713 which inherently has a high Michaelis and Menton, Km, of about 10-2 to 10-3 M for cholesterol and optionally, of cholesterol esterase. The method can measure oxygen consumption, hydrogen peroxide or cholestenone formed. The present invention also provides a test composition for the kinetic determination of cholesterol, comprising cholesterol oxidase with an inherently high Km of about 10-2 to 10-3 M for cholesterol and optionally cholesterol esterase and a system for the determination of hydrogen peroxide, cholestenone or oxygen consumption.

REFERENCES:
patent: 3907642 (1975-09-01), Richmond
patent: 4503144 (1985-03-01), Deeg et al.

Affiliated with

Also associated with

Rating

Method, composition and device for the determination of choleste does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method, composition and device for the determination of choleste, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method, composition and device for the determination of choleste will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-156265