Method and vectors for stabilizing hirudin and human laminin B.s

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 91, 4351723, 4352351, 4353201, 4352523, 43525233, 536 27, 530350, 935 10, 935 29, 935 41, 935 56, 935 61, 935 73, C12P 2102, C12P 1934, C12N 1500, C12N 1700, C12N 121, C07K 300, C07H 1512

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051643049

ABSTRACT:
A method for obtaining heterologous peptides from fusion proteins wherein at least one of the fusion components is connective tissue-activating peptide-III is provided. Hirudin, a laminin B.sub.1 peptide and platelet factor 4 are polypeptides expressed using this method. DNA sequences encoding the fusion protein, vectors containing these sequences and transformed prokaryotic hosts useful in practicing the method of the present invention are also provided.

REFERENCES:
patent: 4828988 (1989-05-01), Bollen et al.
Castor et al, Proc Natl Acad Sci, USA, vol. 80, pp. 765-769 (1983).
Pikkarainen et al, J. Biol. Chem, vol. 262, pp. 10454-10462 (1987).

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