Method and vector organism for controlled accumulation of cloned

Details Method and vector organism for controlled accumulation of cloned Method and vector organism for controlled accumulation of cloned

1987-06-19

1990-08-28

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 712, 4351723, 43525231, 43525233, 435320, C12P 2100, C12N 1500, C12N 120

Patent

active

049525085

ABSTRACT:
A method and a cloning vector are described for the controlled accumulation of cloned heterologous gene products in Bacillus subtilis. The cloning vector is capable of being replicated in B. subtilis and includes the heterologous gene located and oriented such as to be under the control of an operator, promoter, and ribosome binding site sequence. The gene codes for a protein which is under the control of a transport mechanism by which the protein is secreted by the B. subtilis. The gene product is recovered from the growth medium for the B. subtilis. The cloning vector is also capable of similar use in other bacteria such as E. coli.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4666848 (1987-05-01), Gelfand et al.
patent: 4792523 (1988-12-01), Wong et al.
Itakura et al.; Science, 198:1056, (1977).
Yansura et al.; Proc. Natl. Acad. Sci., U.S.A., 81:439, (1984).
Yansura et al. in Genetics and Biotechology of Bacilli, 1984, Ganesan et al. (ed.), Academic Press, Inc., New York, N.Y., pp. 249-263.
Imanaka et al.; J. Bacteriol., 147:776, (1981).
Sherratt et al., Journal of General Microbiology, (1973), 76:217-230.
Taniguchi et al., Proc. Japan Acad., Ser. B, 55:464 (1979).
Gray and Chang, J. Bacteriol., 145:422-428, (1981).
Hori and Osawa, Proc. Natl. Acad. Sci., U.S.A., 76:381-385 (1979).
Keggins et al., Proc. Natl. Sci., U.S.A., 75:1423 (1978).
Erlich, Proc. Natl. Acad. Sci., U.S.A., 74:1680 (1977).
Erlich, Proc. Natl. Acad. Sci., U.S.A., 75:1433 (1978).
Gryczan et al., Proc. Natl. Acad. Sci., U.S.A., 75:1428 (1978).
Duncan et al., Chem. Abstr., 86:185728K (1977).
Kreft et al., Chem. Abstr., 89:103524r (1978).
Rothstein et al., Methods in Enzymology, 68:98 (1979).
Lovett et al., Methods in Enzymology, vol. 69:342 (1979).
Rubin et al., Chem. Abstr., 93:14613p (1980).
Gryczan et al., Molec. Gen. Genet., 177:459 (1980).
Gryczan et al., J. Bacteriol., 141:246 (1980).
Young et al., Genetic Engineering, Chakrabarty (ed.), CRC Press, 1978, pp. 145-157.
Johnson et al., Methods in Enzymol, 65:839-856 (1980).
Kroyer and Chang, Gene, 15:343-347 (1981).
McLaughlin et al., Nucleic Acids Res., 10:3905-3919 (1982).
Wong and Chang, Proc. Natl. Acad. Sci., U.S.A., 83:3233-3237 (1986).
Himeno et al., J. Bacteriol., 168:1128-1132 (1986).

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