Method and vector organism for controlled accumulation of cloned

Details Method and vector organism for controlled accumulation of cloned Method and vector organism for controlled accumulation of cloned

1983-01-26

1987-12-08

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 71, 435 91, 4351721, 4351723, 435231, 435243, 435253, 4353171, 435839, 435849, 536 27, 935 29, 935 41, 935 47, 935 48, 935 52, 935 56, 935 72, 935 73, 935 74, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C12N 1500, C12N 986, C12N 100, C12N 120, C12R 1125, C12R 119, C07H 2104

Patent

active

047118431

ABSTRACT:
A method and a cloning vector are described for the controlled accumulation of cloned heterologous gene products in Bacillus subtilis. The cloning vector is capable of being replicated in B. subtilis and includes the heterologous gene located and oriented such as to be under the control of an operator, promoter, and ribosomal binding site sequence. The gene codes for a protein which is under the control of a transport mechanism by which the protein is secreted by the B. subtilis. The gene product is recovered from the growth medium for the B. subtilis. The cloning vector is also capable of similar use in other bacteria such as E. coli.

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