Method and tissue culture media for inducing somatic embryogenes

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium

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800278, 800298, 435469, 435419, 435430, 4354301, 435431, A01H 400, A01H 500, A01H 510, C12N 1584, C12N 504

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061505870

ABSTRACT:
The present invention relates to improved methods of transforming cacao tissues with Agrobacterium vectors and regenerating transgenic plants. The invention further relates to transgenic cacao somatic embryos and plants obtained according to the methods of the invention. Novel tissue culture media adapted for use in the above-identified methods are also within the scope of the invention. The novel media of the invention include primary callus growth medium, secondary callus growth medium, embryo development medium, primary embryo conversion medium, secondary embryo conversion medium and plant regeneration medium.

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